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Vitamin B 12 serum analysis Mouse serum was diluted at a 1:20 ratio in PBS and holotranscobalamin (holoTC) was measured using an ADVIA Centuar Immunoassay System (SIEMENS) with ADVIA Centuar Vitamin B 12 Test Packs (07847260) according to the manufacturer’s instructions

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Pregnancy detection Stimulating the corpus luteum Fetal development by triggering the production of estrogen and progesterone After ovulation, each follicle that is ruptured and released an egg in the ovary forms a structure called the corpus luteum, which produces progesterone